assay design software  (Sequenom)

Journal: STAR Protocols

Article Title: Protocol to design and implement scalable wireless network system for high-throughput, automated behavioral and circuit neuroscience in mice

doi: 10.1016/j.xpro.2026.104453

Figure Lengend Snippet: PCB design and component overview of the wireless control module (A) PCB layout of the top (left) and bottom (right) layers. Components enclosed by red (a–f), green (g–h), and blue (i–j) boundaries represent passive components, connectors, and active components, respectively. (B) Component list used in the wireless control module.

Article Snippet: Note: Any PCB design software, such as Altium Designer (Altium) or EAGLE (Autodesk), can be used for this procedure.

Techniques: Control

Journal: Nucleic Acids Research

Article Title: Elucidating the kinetics of CRISPR–SaCas9 action to obtain effective HIV DNA excision with two gRNAs

doi: 10.1093/nar/gkag205

Figure Lengend Snippet: Characterization of CRISPR–SaCas9 target site mutations in HIV breakthrough cultures. Supernatants from cultures exhibiting syncytia formation were collected at the peak of infection and used to infect fresh cells transduced with SaCas9/gRNA. After robust viral replication, cells were harvested, and total cellular DNA was extracted. The target region was amplified by PCR and analyzed by Sanger sequencing. Sequences were aligned to the LAI reference, with the wild-type sequence shown at the top and culture numbers indicated on the left. The target sequence is underlined, and the PAM sequence is highlighted in blue. Nucleotide substitutions are shown in red, predicted SaCas9 cleavage sites are indicated with black triangles, and corresponding amino acids are displayed on the right.

Article Snippet: gRNA spacers were designed using Benchling CRISPR guide design software ( https://www.benchling.com/ ), targeting the HIV-1 DNA genome of the primary LAI virus isolate.

Techniques: CRISPR, Infection, Transduction, Amplification, Sequencing